Colchicine poisoning: report of a fatal case with body fluid and post-mortem tissue analysis by high-performance liquid chromatography

1999 ◽  
Vol 13 (3) ◽  
pp. 235-238 ◽  
Author(s):  
Betty Dehon ◽  
Jean-Luc Chagnon ◽  
Elisabeth Vinner ◽  
Jean Pommery ◽  
Daniel Mathieu ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
Body Fluid ◽  
High Performance ◽  
Fatal Case ◽  
Tissue Analysis ◽  
Post Mortem ◽  
Colchicine Poisoning

Detection of Bromisovalum from the Bone Marrow of Skeletonized Human Remains: A Case Report with a Comparison between Gas Chromatography/Mass Spectrometry (GC/MS) and High-Performance Liquid Chromatography/Mass Spectrometry (LC/MS)

1997 ◽  
Vol 37 (3) ◽  
pp. 248-253 ◽  
Author(s):  
H Maeda ◽  
S Oritani ◽  
K Nagai ◽  
T Tanaka ◽  
N Tanaka
Keyword(s):  
Mass Spectrometry ◽  
Bone Marrow ◽  
Gas Chromatography ◽  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Gas Chromatography Mass Spectrometry ◽  
Post Mortem ◽  
Liquid Chromatography Mass Spectrometry ◽  
Chromatography Mass Spectrometry

We report a case of totally skeletonized human remains in which a considerable amount of a hypnotic, bromisovalum (bromovalerylurea), was detected from the bone marrow. The unknown skeletal remains were found in a bush, together with empty vials of bromisovalum and a water bottle. The body was identified as a 46-year-old male, who had died about seven months previously. There was no evidence of trauma. The dried bone marrow of both femurs was separately collected and toxicologically analysed by gas chromatography/mass spectrometry (GC/MS) and high-performance liquid chromatography/mass spectrometry (LC/MS). Bromisovalum was identified from the femurs. The concentration along with the circumstantial evidence suggested possible contribution of bromisovalum to the fatality. However, a considerable quantitative difference was observed between the left and right femur, showing significant post-mortem interference to toxicological evidence. The result showed that multiple sampling at least would be required for quantitative evaluation of post-mortem toxicological evidence. Moreover, the diagnostic value should also be considered with caution in relation to the blood to bone marrow ratio of each drug concentration.

A high-performance liquid chromatography method for hypoxanthine determination in vitreous humour: application to estimation of post mortem interval

2006 ◽  
Vol 25 (5) ◽  
pp. 279-281 ◽  
Author(s):  
J I Muñoz ◽  
E Costas ◽  
M S Rodrĺguez-Calvo ◽  
J M Suárez-Peñaranda ◽  
M López-Rivadulla ◽  
...  
Keyword(s):  
High Performance Liquid Chromatography ◽  
Liquid Chromatography ◽  
High Performance ◽  
Regression Line ◽  
Prediction Method ◽  
Hplc Method ◽  
Post Mortem ◽  
Chromatography Method ◽  
Post Mortem Interval ◽  
A New Technique

We have developed a new technique to determine the concentration of hypoxanthine [Hx] in a reverse phase column using a modified high-performance liquid chromatography (HPLC) method that is faster and more reliable than those previously described. In this paper we present a formula for estimating the post mortem interval (PMI) based on this HPLC method by applying the inverse prediction method. The regression line obtained by changing the variables gives PMI=0.183[Hx]+0.599 (PMI in hours, [Hx] in μmol/L, R2-0.531, P-0.05).

Sign in / Sign up

Export Citation Format

Share Document